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Ete said the poachers entered the sanctuary using a machine boat and shot the animal using a single-barrel gun in an isolated pocket under Borguli Wildlife Range.
She said the accused were booked under various sections of the Wildlife (Protection) Act 1972 and section 25 1(B) of the Arms Act, 1959.
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The seized articles were handed over to the police and a FIR has been registered at Mebo police station, she said.
Sambar deer is a Schedule I species under the Wildlife (Protection) Act, of 1972.
The DFO said stringent action as per the provisions of the Wildlife Act will be taken against any person involved in hunting or abetment of hunting inside the sanctuary.
The informers will be rewarded, she said.